Stabilizing mutations and calcium-dependent stability of subtilisin

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Stabilizing mutations and calcium-dependent stability of subtilisin. Title Stabilizing mutations and calcium-dependent stability of subtilisin. Publication Type Journal Article Year of Publication 2001 Authors Alexander, PA, Ruan, B, Strausberg, SL, Bryan, PN Journal Biochemistry Volume 40 Issue 35 Pagination 10640-4 Date Published 2001 Sep 4 ISSN 0006-2960

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Stabilizing mutations and calcium-dependent stability of subtilisin.

Stability is a property of subtilisin which has proven particularly amenable to enhancement via random mutagenesis and screening, yet the effects of most stabilizing mutations are not understood in structural and energetic detail. This paper seeks to explain the longstanding observation that stabilizing mutations are usually calcium-dependent in their stabilizing effect, irrespective of their p...

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A single calcium binding site is crucial for the calcium-dependent thermal stability of thermolysin-like proteases.

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تاریخ انتشار 2017